Antisense oligonucleotides directed against human ICAM-I RNA

ABSTRACT

The present invention provides a method for the treatment of septic shock and inflammatory complications of shock. A process for selectively inhibiting the expression of the human ICAM-I mRNA transcript using at least one oligonucleotide which is complementary to at least a portion of the human ICAM-I mRNA is disclosed, as are oligonucleotides which are complementary to portions of the ICAM-I mRNA and compositions comprising the oligonucleotides.

RELATED APPLICATION

This application is a Continuation-in-Part of application Ser. No. 07/918,259, filed Jul. 24, 1992, which was abandoned after this application was filed.

BACKGROUND OF THE INVENTION Field of the Invention

This invention relates to agents and therapy to lessen morbidity and mortality by protecting against septic shock, Adult Respiratory Distress Syndrome (ARDS), and other inflammatory complications of shock. Particularly, this invention relates to the treatment of septic shock and the other complications resulting from septic shock by down-regulating the expression of certain cell-cell adhesion receptors or ligands involved in the inflammatory response during septic shock. More specifically, this invention relates to therapy with antisense oligonucleotides which reduce expression of adhesive proteins and protect against septic shock and reduce associated inflammatory damage (like ARDS). Particularly this invention relates to the use of antisense oligonucleotides complementary to human mRNAs or pre-mRNAs coding for ICAM-I (Intercellular Adhesion Molecule-I) to be used in a therapeutic treatment of sepsis (henceforth to include sepsis, septic shock and all other manifestations of the sepsis disease, including but not inclusive of, adult respiratory distress syndrome, multi-organ failure, or cardiovascular dysfunction). Mediators of sepsis produce endothelial dysfunctions that result in the development of an intravascular inflammatory response and subsequent damage to the endothelial cells with migration of leukocytes into the surrounding tissues. This invention also relates to the treatment of sepsis with antisense oligonucleotides targeted to cellular based receptors or their ligands where these receptors or ligands are involved in the inflammatory response during the development of sepsis. This invention further relates to the inhibition of the synthesis of ICAM-I with antisense oligonucleotides.

Description of the Prior Art

Septic shock is defined as a type of shock associated with overwhelming infection. Most commonly, the infection is produced by gram-negative bacteria although other bacteria, viruses, fungi and protozoa may also be causes. As summarized in Infectious Diseases and Medical Microbiology, 2nd edition, edited by Braude et al., Chapter 92, pages 700 et seq.

"Shock is a syndrome of generalized metabolic failure resulting from prolonged inadequacy of tissue perfusion. Its early clinical manifestations reflect malfunction of those organs most dependent on uninterrupted blood flow, particularly the brain, as well as compensatory adjustments designed to maintain adequate arterial pressure. As these adjustments fail, urinary output decreases and biochemical indices of distorted metabolism are detectable; specifically nonoxidative glycolysis with low yield of high energy chemical bonds testifies to the widespread nature of the disorder. In the end, it is the failure of energy production rather than damage to a particular organ that leads to death.

Other terms, such as `circulatory collapse,` `circulatory failure,` and `hypoperfusion,` have been substituted for `shock` in an attempt to pinpoint the specific nature of the derangement. When it occurs as a specific complication of infection, it is referred to as `infectious shock,` `septic shock,` `bacteremic shock,` and even `endotoxin shock.` The last three terms specifically implicate bacterial infection and are therefore too restrictive. Because `infectious shock` is sufficiently broad as well as concise, this term will be used in the present chapter.

Shock may occur in the course of almost any severe infection, but it is particularly characteristic of bacteremia due to gram-negative bacilli. . . . The importance of endotoxin, the lipopolysaccharide (LPS) composing part of all gram-negative cell walls, is readily apparent because it produces a similar syndrome in experimental animals. Partly because of the extensive use of endotoxin as an investigative tool, endotoxin shock is commonly regarded as the prototype of infectious shock."

The shock is believed to be caused by the action of endotoxins, other products of the infectious agent, or host mediators released in response to the infectious agent on the vascular system. Such action causes altered patterns of perfusion of tissues and large volumes of blood to be sequestered in the capillaries and veins.

Sepsis, the sepsis syndrome, and septic shock are not discrete entities, but rather terms that delineate increasingly severe stages of the same disease. Septic shock, a frequently fatal reaction following bacterial infection, has been estimated to occur at a rate of 175 per 100,000 people yearly in the general population and rises to 500 per 100,000 for those people admitted to hospitals (Johnston, J. (1991) J. NIH 3: 61-65). Estimates range up to 400,000 cases of sepsis, 200,000 bouts of septic shock, and 100,000 deaths annually in the United States due to the septic shock induced syndrome (Snell, J. and J. E. Parrillo (1991) Chest 99: 1000-1009). Up to 40-50% of patients who develop septic shock die. The manifestation of septic shock involves a severe decrease in systemic vascular resistance and maldistribution of blood flow. Septicemia, a systemic disease associated with the presence and persistence of pathogenic microorganisms or their toxins in the blood, is currently ranked as the thirteenth leading cause of death in the United States (Annual Summary of Births, Marriages, Divorces, and Deaths: United States, 1988. Hyattsville, Md.: U.S. Department Health and Human Services, Public Health Service, CDC, 1989: 7. Monthly vital statistics report. 1989: 37[13]). Reasons underlying this high incidence of death from septic shock involve increased usage of cytotoxic and immunosuppressive drug therapies which impairs host defense mechanisms or increased use of invasive diagnostic devices or increased patient age (Snell, J. and J. E. Parrillo (1991) Chest 99: 1000-1009). Further causes of impaired host defense mechanisms include diabetes, malignant neoplasms, cirrhosis or extensive burns. The rising rate of infections from organisms other than gram-negative bacteria also contribute to the rise in septic shock induced death. Any bacteria can cause septic shock, however, the gram-negative bacteria (E. coli, Pseudomonas sp. and Bacteroides sp.) in particular evoke septic shock due to the presence of lipopolysaccharide (LPS) in their cell walls. Bacterial LPS, also known as endotoxin, at concentrations as low as a few μg/L can activate immune cells. The majority of damage induced from the presence of LPS is not due to the actual LPS itself, but is in fact a result of the body's complex reaction to the foreign LPS. This response is mediated by immune cell activation and the resultant damage that these activated cells cause to the host tissues.

Septicemia is difficult to reverse and the preferred treatment following the initial signs of hypoperfusion or shock include infusion of normal saline or lactated Ringer's solution. If shock persists then an aggressive fluid challenge is begun and the use of dopamine and/or norepinephrine is recommended. Cardiovascular insufficiency results from alterations to the myocardium and the vasculature and it is myocardial dysfunction that is responsible for hypotension or multiple organ system failure (Snell, J. and J. E. Parrillo (1991). Chest 99: 1000-1009). Unresponsive hypotension usually results from low systemic vascular resistance due to cardiovascular insufficiency which can not be corrected by any therapy. Multiple organ failure usually affects the lungs, kidneys, liver, heart, and central nervous system.

Treatment of septic shock is complex, requiring therapies directed at ameliorating the source of infection [antibiotics], blocking effects of products of the infectious agent and inflammatory mediators on tissues [anti-endotoxin (patent Young et al. U.S. Pat. No. 4,918,163) and anti-cytokine agents (patent Mandell et al. U.S. Pat. No. 4,965,271)], and maintenance of cardiovascular function [volume expansion and pressor agents]. However, mortality still runs at about 100,000 patients per year (40 to 50% of those in shock) and no therapies are available to prevent vascular contractile defects.

Other current approaches to the treatment of sepsis or septic shock involve neutralization of LPS with specific monoclonal antibodies, interference of cytokine-mediated immune responses, or inactivation of cell adhesion proteins with monoclonal antibodies. Targeting of LPS mediated sepsis, however, will be effective only against gram-negative bacteria since LPS is only found in their cell walls. Monoclonal antibodies to the lipid A domain of LPS have had some success at intervention with LPS mediated septic shock from gram-negative bacteria, but not for non-gram-negative induced septic shock (Ziegler, E. J et al. (1991) N. Eng. J. Med. 324: 429-436). Thus, while the gram-negative LPS may be the most potent inducer of sepsis, gram-positive bacterial infections occur in 60-70% of all cases. Intervention with cytokine mediated activation of the immune response as a means of preventing septic shock would not only interfere with gram-negative induced sepsis, but also shock caused by gram-positive bacterial infection or other agents. The development of an effective therapy to treat all bacterial induced septic shock would be of obvious benefit to patients who are at an increased risk of bacterial induced sepsis and provide increased survival from septic shock and the complications that arise during septic shock induced dysfunctions. Another approach would be interference with the cellular response to the various endogenous mediators (cytokines, PAF, arachidonic acid metabolites, histamine, endorphins, etc) responsible for vasculature effects. These approaches are not currently approved for therapy and are in clinical trials.

One of the major effects experienced by the vasculature is destruction of endothelial cells by leukocytes. Inflammation is characterized by the local accumulation of leukocytes, plasma proteins, and fluid usually at an extravascular site. Inflammatory processes are intrinsically destructive to the surrounding tissues and may, in certain circumstances such as allograft rejection or sepsis, be more harmful than beneficial. Thus, an appropriate strategy for the treatment or prevention of sepsis or septic shock would be down-regulation, but not total ablation, of the inflammatory response. Down-regulation of specific cell adhesion receptors and/or ligands to the receptors would be one approach to preventing, or lessening, the inflammatory mediated damage to endothelial cells in the vasculature.

The involvement of the immune response in the development of septic shock and its lethal consequences provides a target that is applicable to the use of antisense oligonucleotides. Antisense oligonucleotides can be used to inhibit expression of the key receptors and cellular ligands involved in the activation of the immune response. The migration of leukocytes into tissues is the central event in the immune or inflammation response. This migration to and subsequent emigration into the tissue is responsible for the successful host response to injury and infection. The leukocytes are also potentially harmful and contribute to the pathology of many inflammatory disorders. The precise mechanism of this injury is not known, but the generation of free oxygen radicals and release of proteolytic enzymes have been implicated and may act together in leukocyte induced endothelial cell damage (Varani, J. et al. (1989), Am. J. Path. 135: 429-436). Evidence for the leukocyte adhesion to endothelial cells has been attributed to specific surface proteins.

Involved in the binding of leukocytes to activated endothelium is a family of endothelial cell adhesion molecules known collectively as the immunoglobin superfamily. One member of this family is the intercellular cell adhesion molecule-1, also known as ICAM-I (Springer, T. A., et al. (1987) A. Rev. Immun. 5: 223-252). ICAM-I is a 90 kD cell-surface glycoprotein of endothelial cells that binds neutrophils and perhaps monocytes via the LFA-I integrin expressed by white blood cells (Kishimoto, T. K. et al. (1989) Adv. Immunol. 46: 149-182). There are two pathways for the adhesion of leukocytes to endothelium: 1) an immediate adhesion that is not dependent upon the de novo synthesis of proteins, and 2) a delayed adhesion (1-2 hours) that is dependent upon the synthesis of proteins (Osborn, L. (1990) Cell 62: 3-6). The synthesis and surface presentation of ICAM-I in endothelial cells suggests that ICAM-I may be involved in the second, or delayed, component of leukocyte adhesion. Although ICAM-I is present at low levels on both epithelium and endothelium under normal conditions, it is markedly upregulated by a variety of inflammatory stimuli such as endotoxin, interleukin-1 and tumor necrosis factor (Prober, J. S. and R. S. Cotran (1990) Transplant. 50: 537-544). Endotoxin or LPS can increase the adhesion of leukocytes to endothelial cells through the biosynthesis and expression of ICAM-I. Stimulation of human umbilical vein endothelial cells (HUVECs) with either interleukin-1, interferon gamma, or tumor necrosis factor-alpha results in more than a 30-fold increase in the surface presentation of ICAM-I (Osborn, L. et al. (1990) Cell 62: 3-6). Also, the stimulation of ICAM-I synthesis by either endotoxin or cytokines requires approximately a two hour delay and then a sustained increased presentation of the endothelial cell surface (Smith, C. W. et al. (1988) J. Clin. Invest. 82: 1746-1756). This two hour delay in presentation of ICAM-I correlated with the time course for adherence of neutrophils (Munro, J. M. et al. (1991) Lab. Invest. 64: 295-299). After 24 hours the expression of ICAM-I and the ability of ICAM-I to be recognized by the antibodies had declined to pre-injection levels (Munro et al. IBID). Thus, this study demonstrates that the early dermal accumulation of neutrophils after injection of endotoxin may be associated with the endothelial cell expression of ICAM-I. These in vivo effects closely parallel the in vitro evidence concerning the induction of ICAM-I by endotoxin and the role of ICAM-I in neutrophil adhesion. The ability of antibodies to ICAM-I to block the adhesion of neutrophils, eosinophils, and basophils induced in vitro in HUVECs by interleukin-1 suggests that ICAM-I plays an important role in the requirement of these cells during the inflammatory response (Bochner, B. S. et al. (1991) J. Exp. Med. 173:1553-1556 and Carlos, T. et al. (1991) Blood 77: 2266-2271).

Activated leukocytes, especially neutrophils, may injure endothelial cells (Pober, J. S. and R. S. Cotran (1990) Transplant. 50: 537-544). While the exact mechanism is not known, the damage may be induced by oxygen radicals or proteolytic enzymes released from the neutrophils. Damage to the endothelial cells lining the vasculature results in leakage into the surrounding tissues. The pulmonary leak that is produced in adult respiratory distress syndrome, a resultant complication of septic shock, most often results from neutrophil-mediated capillary injury (Brigham, K. L et al. (1979) Circ. Res. 45: 292-297).

ICAM-I cDNA and the genomic clones have been isolated and the nucleic acid sequence of the pre- and mature-mRNA can be determined from these sequences (Staunton, D. E. et al. (1988) Cell 52:925-933 and Wawryk, S. O. et al. (1991) Intl. Immunol. 3: 83-93). Thus, the ability to target the pre- or mature-mRNA with antisense oligonucleotides with the express purpose of down-regulating the synthesis ICAM-I is immediately possible.

Involved in the activation of the inflammatory and immune response, as during the development of sepsis and septic shock, is the expression of many essential cell adhesion proteins and receptors. Adhesion molecules are activated by various cellular mediators, exogenous or endogenous to the host, and therefore, the logical approach is down-regulation of adhesion protein expression as opposed to treatments aimed at the multiple activators. Thus, the use of antisense oligonucleotides to specifically down-regulate adhesion protein expression would be of obvious advantage to most therapeutic approaches to septic shock.

Research by others into PKC inhibition and treatment of inflammatory responses have disclosed that endothelial cells express adhesive proteins in response to sepsis associated stimuli such as endotoxin or cytokines, such as interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF). Magnuson, D. K. et al. ((1989) Surgery 106:216-223) and Lane, T. A. et al. ((1990) Biophys. Res. Comm. 172:1273-1281) have shown that these adhesive proteins can be reduced on endothelial cell surfaces by inhibition of PKC with staurosporine or 1-(5-isoquinolinylsulfonyl)-2-methyl piperazine (H7). Surface presentation of these adhesive proteins enhances white blood cell infiltration and activation which can result in tissue damage in inflammatory states like septic shock. In addition, PKC activation enhances endothelial cell permeability resulting in edema. This response to inflammatory agents was also abrogated by exposure of the cells to the PKC inhibitor H7 (Lynch, J. J. et al. (1990) J. Clin. Invest. 85:1991-1998).

Abnormal leukocyte accumulation is implicated in a variety of inflammatory states such as: reperfusion injury, autoimmune diseases, and acute respiratory distress syndrome (ARDS). The damage is thought to result from the release of toxic oxygen radicals and proteases that potentlate tissue damage. The use of anti-adhesive protein antibodies or adhesive like proteins was shown to reduce tissue damage in select models of reperfusion injury (Vedder, N. B. et al. (1988), J. Clin. Inv. 81: 939-944; Simpson, P. J. et al. (1988) J. Clin. Inv. 81: 624-629; Horgan, M. J. et al. (1989) Am. J. Physiol. 259: L315-L319; International patent application of Vadas, M., and M. Berndt (1991) Application # WO 91/07993) and endotoxin induced damage (Rosen, H. and S. Gordon (1989) Br. J. Exp. Path 70: 385-394). When antibodies are used as a treatment they do not control the levels of expression of these proteins and the antibodies typically have short half-lives in circulation. An additional complication of antibodies is the potential for immunogenic reactions to large foreign proteins.

Compared to antibodies, smaller molecules like antisense oligonucleotides can overcome these disadvantages and also provide selective control of expression of a single cellular protein.

The mRNA coding for ICAM-I has been cloned and the nucleic acid sequence is available for selective targeting with antisense oligonucleotides (Staunton, D. E. et al. (1988) Cell 52: 925-933; Wawryk, S. D. et al. (1991) Intl. Immunol. 3: 83-93).

Antisense Background

The use of antisense oligonucleotides for therapeutic purposes was first proposed in 1978 by Stephenson, M. L. and Zamecnik, P. C. ((1978) PNAS 75: 285-288). The concept behind antisense therapy relies on the ability of antisense oligonucleotides to be taken up by cells and form a stable heteroduplex with the target mRNA. The end result of antisense oligonucleotide hybridization is the down regulation of the targeted protein's synthesis. Down regulation of protein synthesis by antisense oligonucleotides has been postulated to result from two possible mechanisms: 1) "hybrid arrest", where direct blocking in pre-mRNA and/or mRNA of sequences important for processing or translation prevents full-length proteins from being synthesized; and 2) an RNase H mediated cleavage and subsequent degradation of the RNA portion of the RNA:DNA heteroduplex (Haeuptle, M. et al. (1986) Nuc. Acids Res. 14: 1427-1448; Minshull, J. and J. Hunt (1986) Nuc. Acids Res. 14: 6433-6451). Thus, the use of antisense oligonucleotides could down-regulate the expression of proteins or enzymes that are implicated in the etiology of a disease state. Down regulation of a protein is functionally equivalent to a decrease in its activity, the mechanism of action of most traditional pharmaceutical drugs.

The interaction of the antisense oligonucleotide with the target mRNA is highly specific as hybridization is determined by the sequence of bases complementary to the antisense oligonucleotide, or by the Watson/Crick base pairing of the two strands of nucleic acid. Thus, there are multiple points of contact between the antisense oligonucleotide and the mRNA target, which increases the specificity for hybridization to the correct sequence. This specificity derived from the Watson/Crick base pairing is not evident in traditional drugs that inhibit the activity of proteins or mimick their action. Potential side effects experienced with traditional drug therapies results through interactions at a few contact points between the drug and various proteins that possess similar binding sites or sites of interaction. Such adverse effects should be eliminated with antisense drugs. Experimental calculations using the number of base pairs in the human genome and the frequency of base utilization predict that there would be a single complement to a 14 mer antisense oligonucleotide in the entire human genome (Ts'o, P. O. P. et al. (1987) Biological Approaches to the Controlled Delivery of drugs, Vol 507, Ann. N.Y. Acad. Sci.). This level of specificity is not achievable with traditional drugs.

Evidence for down regulation of protein synthesis by antisense oligonucleotides has been well documented in vitro (for reviews see van der Krol, A. R. et al. (1988) BioTechniques 6: 958-976; Cohen, J. S. (1991) Antiviral. Res. 16: 121-133). In vivo studies using antisense oligonucleotides have demonstrated that injection of radiolabeled antisense oligonucleotides into the blood of mice results in distribution of full-length labeled oligonucleotide to the various tissues. Once in the tissue, oligonucleotides can elicit an antisense effect by binding to the correct mRNA and, thus, be suitable for a therapeutic approach to a disease state (Miller, P. S. and P. O. P. Ts'o (1987) Anticancer Drug Design 2: 117-128). Recently, antisense oligonucleotides have been shown to elicit a reduction in myc gene expression using an in vivo mouse model for B-cell lymphoma myc expression (Wickstrom, E. et al. (1991) FASEB J. 5: A1442). Other in vivo data has shown that antisense oligonucleotides to 5' vital sequences of tick-borne encephalitis virus were capable of providing protection (30-50% survival in treated animals versus 100% lethality for control mice receiving no antisense oligonucleotide) in mice from viral induced death (Pogodina, V. V., et al. (1989) Dokl-Akad-Nauk-SSSR 308: 237-240). Further evidence for in vivo efficiency of antisense oligonucleotides in mice has shown that subcutaneous injection of a phosphorothioate oligonucleotide could inhibit the synthesis of interleukin-1 receptor protein (Burch, R. and L. C. Mahan (1991) J. Clin. Invest. 88: 1190-1196). The infiltration of neutrophils into the derreal injection site of IL-1 was used as a means of assessing the ability of injected antisense oligonucleotides to inhibit IL-1 receptor synthesis. It was shown that three, 3, nmole subcutaneous injections of phosphorothioate anti-IL-1 receptor oligonucleotides (24 hr intervals) significantly reduced neutrophil infiltration. Control injections of saline or mismatch containing oligonucleotides did not inhibit neutrophil infiltration. Thus, antisense oligonucleotides targeted to the IL-1 mRNA were able to prevent IL-1 induced neutrophil infiltration in mice.

The stability, pharmacokinetics, and bio-distribution of antisense oligonucleotides in vivo have also been reported in mice. Using phosphorothioate antisense oligonucleotides, about 30% of either i.p. or i.v. injected oligonucleotide was retained in the animal with the remaining oligonucleotide being excreted in the urine (Agrawal, S. et al. PNAS 88: 7595-7599, 1991). The oligonucleotide retained within the animal was found within most tissues up to 48 hours. There were differences in the rates of degradation within the various tissues, with the plasma, stomach, heart and intestine showing the most intact oligonucleotide (15% degraded). In the kidney and liver degradation was 50% or greater after 48 hours. All of these experiments demonstrate that the antisense oligonucleotides are capable of reaching tissues in vivo and that the expression of gene products, proteins derived from mRNA translation, can be specifically down-regulated by antisense oligonucleotides in live animals.

A major issue to be addressed in the use of antisense oligonucleotides for in vivo treatment is the stability of the molecule to the action of nucleases. Use of unmodified oligonucleotides containing phosphodiester linkages has not proven valuable in antisense therapy since these oligonucleotides are susceptible to exo- and endo-nucleases present in serum and cells. Thus, modifications of the natural, or phosphodiester deoxyoligonucleotides (PO-ODN) have been developed that provide increased stability to degradation (for review Uhlmann, E. and A. Peyman (1990) Chemical Reviews 90: 543-584). One of these modifications is the replacement of one of the non-bridging oxygen atoms in the phosphodiester linkage with sulfur to produce a phosphorothioate deoxyoligonucleotide (PS-ODN). The introduction of sulfur atoms in the PS-ODNs do not disrupt hybridization significantly compared to unmodified PO-ODNs, retains the relative solubility of PO-ODNs in aqueous media and provides significantly enhanced resistance to serum and cellular nucleases (Stein, C. A. et al. (1988) Nuc. Acids Res. 16: 3209-3221; Campbell, J. M. (1990) J. Biochem. Biophys. Methods 20: 259-267). Other modifications include methylphosphonates, phosphorodithioate, sugar modifications, and heterocycle modifications (Goodchild, J. (1990) Bioconjugate Chem 1: 165-186). Of various modifications to oligonucleotides, the PS-ODNs are suitable for antisense approaches to in vivo therapy at the present time; however, as novel chemistries become available there may be many new chemical modifications of antisense oligonucleotides that have increased potential for therapeutic applications.

The bio-availability of antisense oligonucleotides to target cells is another important issue that arises in the use of oligonucleotides for therapy. Enhanced cellular uptake has been reported using a wide variety of techniques including LIPOFECTIN™ (Life Sciences Technology, Gaithersburg, Md.) (Chiang, M-Y. et al. (1991) J. Biol. Chem. 266: 18162-18171), lipoproteins (de Schmidt, P. C. (1991) Nuc. Acids Res. 19: 4695-4700), and a wide variety of conjugates, including poly-L-lysine or cholesterol etc. ( for review see Goodchild, J. (1990) Bioconjugate Chem. 1: 165-186). The ability to increase the uptake of ODNs into cells will become more diverse as more data accumulates. Conjugation of cholesterol to the 5' end of an oligonucleotide resulted in a molecule that exhibited reduced serum clearance, due to reduced kidney excretion, compared to control ODNs (de Smidt, P. C. et al. (1991) Nuc. Acids Res. 19: 4695-4700). This conjugation of cholesterol to ODNs may result in increased delivery to liver cells via the LDL transport mechanism. Other possibilities for increasing cellular uptake include modulation of the 80 kDa surface protein putatively involved in transport of oligonucleotides into cells. The availability of more information on this protein may provide insight into certain modifications that increase the activity of the transporter (Loke, S. L., et al. (1989) Proc. Natl. Acad. Sci. USA 86: 3472-3478). The conjugation of ODNs to certain biological molecules that are taken up into cells by specific receptors may provide an in vivo advantage in antisense oligonucleotide delivery. For example, conjugation of folate to the oligonucleotide may provide a selective cellular uptake mechanism through the endocytosis mediated vitamin folate pathway (Leamon, C. P. and P. S. Low (1991) Proc. Nat. Acad. Sci. USA 88: 5572-5576). Conjugation of folate to antibodies has demonstrated that this approach with large macromolecules is feasible. Thus, conjugations, such as folate or other ligands recognized by receptors, or to agents that carry the ODNs into cells via endocytotic uptake pathways, may be an extremely useful approach to antisense therapy. There may also be other conjugations that would target oligonucleotides to specific cells or organs which would decrease the effective concentration of oligonucleotide needed in a specific therapy. However, present data suggests that antisense oligonucleotides with modifications like phosphorothioate or methyl phosphonate are capable of tissue distribution and cellular uptake. Thus, at the present time an oligonucleotide sufficiently stable to nuclease degradation should be distributed to the cells and undergo cellular uptake.

Recently, it has been reported that antisense PS-ODNs are capable of down-regulating the in vitro expression of one member of the immunoglobin superfamily adhesion proteins involved in cell--cell adhesion, ICAM-I (Chiang, M-Y. et al. (1991) J. Biol. Chem. 266: 18162-18171). These data suggest that antisense oligonucleotides, particularly those stable to nucleases, like the phosphorothioate or 2'-O-methyl modified antisense oligonucleotides, are capable of inhibiting the expression of cell adhesion molecules. Thus, the use of antisense oligonucleotides in the down-regulation of ICAM-I and other adhesion molecules should provide a basis for therapy and treatment of inflammatory disorders, particularly septic shock. Inhibition of the inflammatory response component of sepsis may provide the necessary protection to prevent the lethal consequences of septic shock brought about by the body's own immune system.

While there are several generalized locations within the linear nucleotide sequence of any mRNA that may provide an increased capacity for antisense oligonucleotide intervention with the flow of information from the gene to the protein product, there is, as of yet, no way to predict the optimum sequence for an active antisense oligonucleotide. Uhlman and Peyman (1990) discuss the potential mechanisms of action for antisense oligonucleotides as inhibition of splicing, polyadenylation, translocation, translation and ribosome movement along the mRNA but the actual selection of a particular sequence is only discussed in general terms. One would select antisense oligonucleotides that target nucleotide sequences anywhere in a particular mRNA (i.e. 5'-untranslated region, coding region, 3'-untranslated region, or combinations thereof) because one wants to inhibit the expression of that mRNAs protein. One would not want to select an antisense sequence that is complementary to one protein's mRNA when the objective is to inhibit the expression of another protein. However, the selection of an antisense sequence, anywhere (whether located in the 5'-untranslated region, coding region, 3'-untranslated region, or combinations thereof) within the nucleotide sequence of the target protein's mRNA will not always produce active antisense compounds.

Recent literature shows a number of specific examples of very potent in vivo therapeutic efficacy for antisense oligonucleotides. The examples cited below show three different therapeutic targets that indicate the wide range of therapeutic activity for antisense oligonucleotides.

The first study (Simons, M. et al., 1992, Nature 359:67-70) shows that an antisense phosphorothioate directed against c-myb can be delivered in vivo to rat carotid arteries stripped of their intima. The oligonucleotides were formulated in a pluronic gel containing 200 micrograms of drug and applied to the outer layer of the arteries. The drug had to reach the smooth muscle cells surrounding the intimal layer, in order to have a therapeutic effect. Indeed, the antisense oligonucleotide effectively prevented restenosis or regrowth of smooth muscle cells around the stripped regions. This effect indicates strongly that antisense can be therapeutically effective in living rats in a stringent model.

A second study shows that a human leukemia can be treated successively in a scid mouse model with antisense oligonucleotides against c-myb (Ratajczak, M. Z. et al, 1992, Proc. Natl. Acad. Sci. USA 89:11823-11827). This work established human leukemia-scid mouse chimeras with K562 cells and treated diseased animals with phosphorotioate-modified antisense oligonucleotides against c-myb. The results showed that control oligonucleotides had no effect on survival, whereas animals treated with antisense constructs survived at least 3.5 times longer than the various control animals. Antisense treated animals had significantly less disease at sites where leukemia is often refractory to treatment, the brain and the ovary. The drug was delivered to the animals by Alzet minipumps that released 1.0 microliter per hour into a paraspinal pocket at a 100 microgram per day so that each animal received 5 mg/kg or 1.0 micromolar dose. Thus, based on the results of this animal model, it seems quite reasonable that antisense may have efficacy in this and other cancer models.

A third study shows that antisense oligonucleotides to the NMDA-R1 receptor channel can protect cortical neuron of rats from excitotoxicity and reduce focal ischemic infarctions (Wahlestedt, C. et al., 1993, Nature 363:260-263). In this rat model of stroke, antisense therapy was more effective than MK-801, the most effective small molecule therapy for stroke. The antisense drug was delivered intraventricularly in a dose of 15 nmol in 5 ul H₂ O. The appropriate controls had no effects.

In summary, these data show that antisense therapy applied to stringent animal models of human disease can cause profound therapeutic benefits in the models. One can thus say that, while there is more in vitro antisense data, the efficacy seen in these animal models is sufficient to predict that there may be profound therapeutic benefit in humans from antisense drugs discovered in vitro. Of course this is precisely the situation for standard small molecule drugs discovered in vitro.

Investigations into the toxicity of antisense oligonucleotides has not revealed significant damage or lethality to cells. In vitro studies into the toxicity of antisense oligonucleotides has been limited primarily to modified oligomers where the phosphodiester linkages between the sugar moieties has been replaced with either phosphorothioates or methylphosphonates. Generally, phosphorothioates are non-toxic in a variety of cell lines (for example, Reed, J. C. et al. J. Cohen Cancer Res. 50: 6565-6570, 1990).

SUMMARY OF THE INVENTION

Accordingly, an object of the invention is a therapy to lessen morbidity and mortality caused by septic shock and the ensuing complications and associated symptoms.

An additional object of this invention is a method of treating the symptoms of septic shock.

Yet another object of this invention are agents to prevent vascular and tissue defects associated with manifestations of septic shock.

Another object of this invention is a formulation of an inhibitor of the inflammatory response that is a major cause of the complications resulting in septic shock.

An additional object of this invention is a method for inhibiting the adhesion of leukocytes to the endothelium of the vasculature, where the adhesion of leukocytes to the endothelium is the initial event in the development of the inflammatory response.

The foregoing objects of the present invention are accomplished by providing an improved therapeutic treatment for the collective diseases of `sepsis` that result from inflammatory response mediated changes in the vasculature by using nucleic acids, either as oligonucleotides or polynucleotides, as antisense inhibitors of protein translation, specifically ICAM-I.

These and additional objects of the invention are accomplished by treating the subject susceptible to septic shock with antisense oligonucleotide, preferably with a sequence which inhibits synthesis of the protein ICAM-I. Preferred are those oligonucleotides which substantially form a stable hybrid with at least a portion of the gene or mRNA coding for ICAM-I. Preferred is an oligonucleotide that comprises a sequence that forms a stable hybrid with at least a portion of the 5' UTR (UnTranslated Region), translation initiation codon, coding region, or 3' UTR of the pre- or mature- mRNA for ICAM-I. Other preferred sites include the remaining regions of the exons of the gene and the intron regions to inhibit pre-mRNA processing. The inhibitors of this invention are administered, preferably by intravenous infusion in a suitable pharmaceutical carrier, in a range of 0.01 to 500 mg/Kg body weight preferably in the range of 0.05 to 250 mg/Kg body weight and most preferably in the range of 0.15 to 50 mg/Kg body weight.

In preferred embodiments, these antisense oligonucleotides comprise a nucleotide sequence that forms heteroduplexes within the 5' untranslated region of the mRNA, the translational initiation codon, the sequence immediately downstream from the translation initiation codon, unique portions of the Y untranslated regions, or combinations thereof. These oligonucleotides may comprise from a 10 to a 30 mer.

The oligonucleotide can be used to inhibit the expression of ICAM-I. Thus, the invention encompasses the use of at least one oligonucleotide, alone or in combination in the therapeutic treatment of patients suffering from septic shock or the resultant complications from sepsis.

In a preferred embodiment of the invention, a therapeutically effective concentration of oligonucleotide can be administered to a patient so as to substantially block the translation of the human ICAM-I mRNA, thereby substantially reducing the complications of sepsis.

The present invention also provides a pharmaceutical composition comprising one or more oligonucleotides of the invention in combination with a suitable pharmaceutical carrier according to conventional pharmaceutical techniques.

BRIEF DESCRIPTION OF THE DRAWINGS

A more complete appreciation of the invention will be readily obtained by reference to the following Description of the Preferred Embodiments and the accompanying drawings in which like numerals in different figures represent the same structures or elements. The representations in each of the figures is diagrammatic and no attempt is made to indicate actual scales or precise ratios. Proportional relationships are shown as approximations.

FIG. 1 is a map of ICAM-1 molecule showing specific oligonucleotides of this invention.

FIG. 2 is predicted secondary structure for 5'-untranslated end (nucleotides 1-300) of human ICAM-1 (Free Energy for formation=-80.2 kcal/mole)

DESCRIPTION OF THE PREFERRED EMBODIMENTS

In accordance with the detailed description, the following definitions apply:

Antisense oligonucleotides--any natural or modified oligonucleotide or chemical entity that binds specifically to a pre-mRNA or mature mRNA which results in interference or inhibition with translation of the mature mRNA or prevents the synthesis of the polypeptide encoded by the mature mRNA.

Septic shock--septic shock is used in this invention to convey any of the various states of the collective diseases known as sepsis, sepsis syndrome, septic shock, incipient septic shock, bacteremia, refractory septic shock, or any of the diseases associated with sepsis such as ARDS (adult respiratory distress syndrome), multiple organ failure, cardiac dysfunction, etc.

ICAM-I--ICAM-I in this invention describes the endothelial cell's Intercellular Adhesion Molecule -I involved in adherence of leukocytes (monocytes and neutrophils especially).

mRNA--mRNA refers to mature, processed mRNA or unprocessed, nuclear pre-mRNA (ribonucleic acid) to the ribonucleic acid transcribed from the gene(s) encoding for the synthesis of ICAM-I. These sequences of ribonucleic acid are used to select the antisense oligonucleotide sequences which are complementary to discrete portions of the mRNA or pre-mRNA.

As used herein, unless otherwise indicated, the term "oligonucleotide" includes both oligomers of ribonucleotides i.e. oligoribonucleotides, and oligomers of deoxyribonucleotides i.e. oligodeoxyribonucleotides, or oligodeoxynucleotides.

Unless otherwise indicated, the term "oligonucleotide" also includes oligomers which may be large enough to be termed "polynucleotides".

The terms "oligonucleotide" and "oligodeoxynucleotide" include oligomers and polymers of biologically significant nucleotides, adeninc, deoxyadenine, guanine, deoxyguanine, thymine, uracil, cytosine and deoxycytosine, as well as oligomers and polymers which contain other novel nucleotides and are hybridizable to the target mRNA transcript. These terms also include oligomers and polymers having one or more purine or pyrimidine moieties, sugar moieties, or internucleotide linkage(s) which has or have been chemically modified. Such modifications may be substantial and may encompass nonnucleotide chemistries including non-sugar, non-phosphate backbone, and chemical alterations to the bases to maintain the specific hybridization to the mRNA by base-pairing mechanisms, similar to or different from Watson-Crick base pairing. These terms further include those oligomers or polymers that are composed of nucleoside containing bases joined to the sugar moieties in the alpha configuration.

The term "downstream" is used herein to indicate the 5' to Y direction in a nucleotide sequence. Similarly, the term "upstream" indicates the 3' to 5' direction.

The term "complementary" is used herein to indicate that the oligonucleotide is capable of hybridizing to and forming a duplex with its target sequence in the mRNA transcript.

The term "stable duplex" or a "stable hybrid" is used herein to indicate that 50% or greater of the oligonucleotide is bound in said duplex with its target sequence in the mRNA transcript at a temperature of 40° C. in 10 mM sodium phosphate, 1 mM ethylenediamine tetraacetic acid and 128 mM sodium chloride, pH 7.

The term "mRNA" is used herein to indicate either the mature or processed mRNA, or the unprocessed nuclear pre-mRNA.

According to a preferred embodiment of the present invention, antisense oligonucleotides are synthesized using standard published techniques for the synthesis of phosphorothioate, PS, oligonucleotides, ODNs. Synthesis of antisense oligonucleotides is performed using a solid support and a commercially available DNA synthesizer. Antisense oligonucleotides are synthesized using standard phosphoramidate chemistry. For phosphodiester linkages the oxidation is mediated via iodine, while for the synthesis of phosphorthioates, the oxidation is mediated with a 0.2M solution of 3H-1,2-benzodithiole-3-one, 1,-dioxide in acetonitrile (Iyer, R. P., et al. (1990) J. Amer. Chem. Soc. 112:1253-1254) for the step-wise thioation of the phosphate linkages. The thioation step is increased to 68 sec and is followed by a capping step. Following synthesis and cleavage from the control pore glass support, the trityl-on oligonucleotide is purified using HPLC. HPLC methodologies consist of chromatography using an PRP-1 column and gradient of acetonitrile in 50 mM triethylammonium acetate, pH 7.0 (4-32% in 30 min, flow rate of 1.5 ml/min). Appropriate fractions are pooled, evaporated, treated with 5% acetic acid for 15 min at ambient temperature. The oligonucleotide solution is extracted with an equal volume of ethyl acetate, neutralized with ammonium hydroxide, frozen and lyophilized. Solution based chemistries are also useful for synthesis of antisense oligonucleotides and are useful for scaled-up synthesis of oligonucleotides.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is concerned with oligonucleotides having therapeutic value, therapeutic compositions containing these oligonucleotides and methods for use thereof. The present invention is the result of research on septic shock and the role of inflammation in the resultant morbidity or mortality resulting from septic shock. Accumulated information has led to the conclusion that disruption of the inflammatory response would be a method of choice in the treatment of septic shock and the resulting complications.

It has previously been demonstrated in a number of systems by various investigators that oligonucleotides complementary in sequence to a portion of a particular mRNA are capable of hybridizing to the rnRNA and inhibiting the translation of the transcript.

In accordance with the present invention, an oligonucleotide having a base sequence capable of hybridizing to the mRNA transcript of the human ICAM-I adhesion receptor is provided. Hybridization of the oligonucleotide to the ICAM-I mRNA substantially blocks the translation of the mRNA transcript. Because ICAM-I is essential for the initial attachment or adhesion of leukocytes arising from stimulations that induce septic shock, down-regulation of ICAM-I expression would provide a benefit to the patient in the form of a reduced inflammatory response.

The oligonucleotides of the present invention are constructed and purified by methods known in the art. The specific oligonucleotide sequence is complementary to a portion of ICAM-I mRNA or gene. In particular, the oligonucleotide sequence is complementary to a portion of ICAM-I transcript, such as the portion of the transcript including the translation initiation codon, and sequences 5' and/or 3' to the translation initiation site, of the 5' cap region of the mRNA and sequences 3' to the cap site. Another oligonucleotide sequence is also made complementary to a portion of the 3' untranslated region of the ICAM-I gene that is substantially unique to the ICAM-I gene. Moreover, antisense oligonucleotides that are capable of cross-linking DNA or intercalating DNA can be made complementary to any portion of the ICAM-I gene. Furthermore, the invention contemplates that any oligonucleotide or combination of oligonucleotides capable of specifically and substantially inhibiting the expression of ICAM-I can be used.

The oligonucleotides of this invention comprise predetermined sequences of DNA ranging in size from about 10 bases up to about 30 bases, which is sufficient to define a unique sequence in the human ICAM-I target mRNA transcript. Alternatively, two or more oligonucleotides of 7-15 bases may be joined together by non-nucleotide linkages. For such oligonucleotides less than 14 bases may be used, however the degree of sequence uniqueness decreases rapidly with decreasing length and thereby greatly reduce the specificity of the oligonucleotide for the ICAM-I mRNA transcript. On the other hand, oligonucleotide sequences greater than about 30 bases may be subject to decreased cellular uptake and have an increased likelihood of containing short stretches of nucleotide sequence that is capable of forming quasi-stable hybrids with non-target mRNA sequences, other than the ICAM-I mRNA transcript. It is preferable that the oligonucleotides comprise about 15 to 25 bases. In the most preferred embodiment of this invention, a 21 mer oligonucleotide is used, to be selected from the group of oligonucleotides consisting of at least GCT TTC CCG GAA ACC TCG CGC (SEQ ID No. 1); GGG GCG GTG CTG CTT TCC CGG (SEQ ID No. 2); AGC CAC CTG GGG GCC AAG GGG (SEQ ID No. 3); TGA TCC TTT ATA GCG CTA GCC (SEQ ID No. 4); GCG TCG ACT GGG GCG CGT GAT (SEQ ID No. 5): CTC TGA GTA GCA GAG GAG CTC (SEQ ID NO. 6); CCC CGA CTC ACC TGG GAA CAG (SEQ ID No. 7); GCT ACA CAT GTC TAT GGA GGG (SEQ ID No. 8); GGC AGA AAT GTA TGT GGG TGG (SEQ ID No. 9); ACA CAT ACA CAC ACA CAC ACA (SEQ ID No. 10); AGC CAT AGC GAG GCT GAG GTT (SEQ ID No. 11); GGT TGC AAC TCT GAG (SEQ ID No. 12); GCC ATA GCG AGG CTG A (SEQ ID No. 13); CTA TGG AGG GCC ACT TC (SEQ ID No. 14), and GGG CCA GIT CTT CTG TAA (SEQ ID No. 15) wherein the sequences as listed are each in the 5' to 3' direction.

The oligonucleotide sequence may be prepared by solid phase or solution phase synthesis in an automated nucleic acid synthesizer or via solution phase techniques. Also, in a less preferred method, the oligonucleotide may be prepared through the use of reverse transcriptase, PCR synthesis, or via other genetic engineering techniques. The method of preference for this invention is synthesis of the oligonucleotide using automated DNA synthesis on a solid phase support. Any means of synthesis of the oligonucleotides is within the scope of this invention.

Modifications to the oligonucleotides may be used to increase several desirable properties, including solubility, enhanced uptake, or enhanced stability to degradation, for example. Thus, modifications to the phosphate backbone, termini, sugar moiety, or the individual nucleic acid bases are within the scope of this invention. A preferred modification to the oligonucleotides is the alteration of the phosphodiester linkage between the sugar moieties. A more preferred embodiment of this invention is the use of phosphorothioate linkages between the sugar moieties. The combination of various modifications, for example, phosphate backbone modifications in combination with any number of terminal conjugates, is within the scope of this invention. The terminal modifications may include cross-linking agents, intercalators, photochemically activated moieties, alkylating agents and redox active nucleic acid cleavage groups. Also included are modifications that are nonnucleotide based in chemistry but that still form stable heteroduplexes with at least a portion of the ICAM-I mRNA. For example, non-nucleotide i.e. (CH₂)_(n) linkers between oligonucleotides are included.

The composition of the present invention comprises at least one oligonucleotide having a nucleotide sequence at least substantially complementary to at least a portion of the human ICAM-I mRNA transcript in a suitable pharmaceutical carrier. The amount of oligonucleotide in the composition can range from about 0.01% to 99% by weight of the composition. The oligonucleotide can be mixed with a variety of carrier compounds depending upon the form of the preparation desired for administration, e.g. delivery by i.v modalities. The more preferred means of delivery is via an i.v. solution prepared with any of the usual pharmaceutical components.

The selection of antisense oligonucleotides that exhibit activity against a particular mRNA requires the determination of active antisense oligonucleotides experimentally. For example, data obtained from evaluating the capacity for a series of antisense oligonucleotides targeting the mRNA for Intercellular Adhesion Molecule-1 (ICAM-1) to inhibit expression of the protein product revealed that there were no generalized rules for the selection of the antisense oligonucleotides that exhibited optimum or even sub-optimum activity (Table 1). The mRNA for ICAM-1 is 3,058 nucleotides in length. The length of the 5'-untranslated region is 123 nucleotides, the coding region 1,593 nucleotides long, and the 3'-untranslated region is 1,342 nucleotides in length. Assuming that one wishes to design antisense oligonucleotides 21 nucleotides in length there are 3,038 potential 21 mers that could be synthesized. However, many of these 3,038 oligonucleotides will not exhibit sufficient activity to inhibit translation of the ICAM-1 mRNA. Experimental determination of the capacity for 40 different antisense oligonucleotides to inhibit ICAM-1 protein synthesis revealed that approximately 35% of the synthesized oligonucleotides did not significantly inhibit ICAM-1 protein synthesis (greater than 50% inhibition at 0.1 μM) as depicted in FIG. 1 where the long bar denotes the mRNA sequence of human ICAM-1. The 5'cap, untranslated regions (UTR), start codon (AUG), coding sequence, termination codon (UAA) and polyadenylated tail are enumerated above the bar. Gray areas denote intron regions. The shorter bars denote oligodeoxynucleotides (ODN) that have been evaluated for their ability to suppress expression of ICAM-1 in human umbilical vein endothelial cells. The code number for each ODN is printed just below the bars. All ODNs were evaluated at 0.1 μM. Bold bars represent ODNs that inhibit ICAM-1 expression greater than 50%

In these experiments, antisense oligonucleotides were added to human umbilical vein endothelial cells (HUVEC) in the presence of 10 μg/ml Lipofectin (Life Technologies, Gaithersburg, Md.) in serum free media for 4 hours. Media containing oligonucleotide was removed and media containing 10% fetal calf serum was added.

                  TABLE 1                                                          ______________________________________                                         ICAM-1 ANTISENSE OLIGONUCLEOTIDES                                              Oligo-           Length                %                                       nucleotide.sup.1                                                                       Location.sup.2                                                                          (Nucleotides)                                                                             % GC.sup.3                                                                           Tm.sup.4                                                                            Inhibition.sup.5                        ______________________________________                                         GM1594   85-105  21         63.4  54.5 81                                      GM1595  109-129  21         61.9  71.8 80                                      GM1786  113-128  16         62.5  59.4 67                                      GM1824  73-93    20         65.0  67.5 17                                      GM1826   82-103  22         54.5  63.4 70                                      GM1827  116-133  18         66.7  68.5 62                                      GM1828  107-126  20         60.0  67.5 61                                      ______________________________________                                          .sup.1 Oligonucleotides  modified using phosphorothioate chemistry.            .sup.2 Location  represents the number of nucleotides in the mRNA that ar      complementary to the antisense sequence.                                       .sup.3 % GC  represents the percentage of guanine and cytosine nucleobase      comprising the total nucleotide composition of the oligonucleotide.            .sup.4 Tm  represents the predicted Tm (represents the temperature,            degrees C., where the strands are half dissociated or denatured). These        values are predicted using the nearest neighbor approach (Breslauer et         al., 1986, Proc. Natl. Acad. Sci. 83: 3746-3750 and Freier, et al., 1986,      Proc. Natl. Acad. Sci. 83: 9373-9377).                                         .sup.5 % Inhibition  represents the percent decrease in IL1β (10          U/ml)induced ICAM1 protein expression. Cells (human umbilical vein             endothelial) were treated with 100 nM oligonucleotide prior to ICAM1           induction. ICAM1expression was determined 24 hours postinduction.        

Approximately 16 hours later, ICAM-1 protein synthesis was induced by the addition of either human tumor necrosis factor-alpha (hTNF-α at 40 U/ml, IL-1β at 10 U/ml, or LPS (lipopolysaccharide at 0.5 μg/ml). These compounds caused marked induction of ICAM-1 protein expression as determined by a radio-immuno assay. Evaluation of the data demonstrated that there were no rules or obvious sequence locations that consistently predict active antisense oligonucleotides. For example, evaluation of a series of phosphorothioate-modified oligonucleotides directed against the ICAM-1 mRNA sequence between nucleotides 73-112 exhibited variable potencies for inhibiting the expression of the ICAM-1 protein (from 0-80% inhibition at 0.1 μM oligonucleotide). Oligonucleotide GM1595, a 21 mer, inhibited hTNF-α-induced expression 80% compared to hTNF-α-stimulated control cells (receiving a mixed sequence oligonucleotide). GM1595 was designed to hybridize to ICAM-1 mRNA nucleotides 109-129 (the translation initiation codon is at nucleotides 124-126). Other oligonucleotides that hybridize to the translation initiation codon were assayed to determine their capacity to inhibit ICAM-1 expression. Three of these oligonucleotides exhibited a capacity to inhibit ICAM-1 expression. The 16 mer GM1786 (complementary to nucleotides 113-128), the 18 mer GM1827 (complementary to nucleotides 116-133), and GM1828, a 20 mer (complementary to nucleotides 107-126) exhibited reduced potency at 0.1 μM (67%, 62% and 61% inhibition, respectively). That oligonucleotides capable of hybridizing to the translation initiation codon were active is not unexpected based upon literature data. All four oligonucleotides were designed to hybridize to the only characterized semi-conserved translation regulation sequence located near the initiation codon, the Kozak sequence (Kozak, M., 1987, J. Mol. Biol. 196: 947-950). The differences observed with these four oligonucleotides (inhibition ranged from 61 to 81%) could not be predicted based upon the analysis of their ability to form hybrids with the mRNA. Examination of the predicted Tm (based upon the nearest neighbor approach) demonstrates that GM1786 possesses the lowest Tm (59.4° C.) while more active oligonucleotides GM1827 and GM1828 are predicted to have higher Tms (68.5° and 67.5° C., respectively) even though they are less active.

Another series of oligonucleotides that were designed to hybridize between nucleotides 73-112 also contained several active oligonucleotides. These oligonucleotides are upstream from the translation initiation codon and were not selected based upon sequence location. GM1594 (spanning nucleotides 84-104) exhibited 81% inhibition of hTNF-α-induced ICAM-1 expression. However GM1824, complementary to nucleotides 89-104, only produced a 24% inhibition of ICAM-1 expression. To evaluate if the antisense activity resides in the capacity of the oligonucleotide to hybridize to mRNA sequences between 84-89, two other oligonucleotides were evaluated. GM1826 (complementary to nucleotides 82-103) and GM 1824 (complementary to nucleotides 73-93) inhibited hTNF-α-induced ICAM-1 expression 67% and 17%, respectively. The reason for this variability in activities is not readily apparent. Comparisons of the predicted Tms for these four antisense oligonucleotides do not suggest that some would be active while others are relatively inactive. FIG. 2 shows the secondary structure [predicted by computer using data provided by M. Zuker, 1989, Science, 244: 48-52, and J. A. Jaeger, D. H. Turner and M. Zuker, 1989, Proc. Natl. Acad. Sci. USA 86: 7706-7710] that would be present in nucleotides 1-300 for the 5' end of ICAM-1 mRNA. All four of the oligonucleotides are complementary to sequences contained within a predicted stem-loop structure that encompasses nucleotides 69-128. These oligonucleotides exhibited a range of Tms (45.0°-63.4° C). However, GM1785 (Tm 59.4° C.) was the least active (24% inhibition) while GM1594 (Tm 54.5° C.) was the most active (81% inhibition). Again, neither sequence location, location in secondary structures, or their capacity to hybridize with mRNA were predictive of active oligonucleotides.

Based upon the above discussions of antisense activity for oligonucleotides that are similar in length and complementary to the same nucleotides, there are no rational explanations or rules that would predict active sequences. There have been suggestions that mRNA secondary structures might be important factors that would make the selection of an active antisense oligonucleotide obvious. The predicted free energy for this structure is -80.2 kcal/mole. There does not appear to be any obvious feature of the mRNA secondary structure that would suggest that oligonucleotides complementary to any sequence would exhibit antisense activity.

Based upon the above discussion of the data obtained in the analysis of antisense oligonucleotides targeting the mRNA for human ICAM-1, there are no obvious regions of a target mRNA that can be predicted to be effective targets for antisense oligonucleotide induced down regulation of protein synthesis. The selection of any specific oligonucleotide sequence that exhibits antisense activity requires rigorous experimental evaluation of a number of antisense sequences. The experimental determination for ICAM-1 antisense sequences suggest that there are only generalized regions of the mRNA for selecting an active antisense oligonucleotides that can significantly inhibit the expression of a particular protein. Thus without any rules or generalizations for predicting active antisense oligonucleotides, many different regions of the human ICAM-1 mRNA must be experimentally examined for selection of active antisense sequences. After analysis of this data only those oligonucleotides that exhibit significant inhibition of ICAM-1 protein expression are selected for further analysis.

The following examples illustrate the principles and practices of the invention and are not intended to limit its scope in any way.

EXAMPLE 1

This example describes the preparation and use of a series of oligonucleotides whose base sequences are complementary to sequences contained in the human ICAM-I mRNA transcript. The oligonucleotides are synthesized on an automated DNA synthesizer using standard techniques practiced in the art.

Phosphorothioate oligonucleotides are synthesized using standard procedures (Iyer, R. P. et al. (1990) J. Amer. Chem. Soc. 112: 1253-1254). A sequence of one of these phosphorothioate oligonucleotides, complementary to the 5' untranslated region and translation initiation codon, is 5'-AGC CAT AGC GAG GCT GAG GTT-3' (SEQ ID No. 11), where the translation initiation codon is underlined and in bold face type. This oligonucleotide is known by the name GM1595. GM1595 is prepared for delivery to the vasculature using an i.v. solution containing the oligonucleotide. The antisense oligonucleotide is presented to the lining of the vasculature (the endothelium cells expressing ICAM-I) taken up by the cells and forms a stable heteroduplex with the mRNA transcript that codes for ICAM-I. This hybridization of the oligonucleotide to the mRNA results in a down-regulation of ICAM-I synthesis. Due to the down-regulation of ICAM-I synthesis, there is a reduction in the cell membrane presentation of ICAM-I. With a down-regulation of ICAM-I there is a reduction in the adhesion of leukocytes to the endothelium, and thus, a reduction in the inflammatory response.

The frequency and duration of i.v. oligonucleotide therapy, concentration of oligonucleotide in the i.v. fluid will vary from patient to patient and can be determined by the prescribing physician. While the inflammatory response is being abated by the antisense oligonucleotide therapy, the use of standard therapies are used to control the bacterial or other infection that resulted in the manifestation of the septic shock.

EXAMPLE 2

The inhibitors of this invention are administered, preferably by intravenous infusion in a suitable pharmaceutical carrier, in a range of 0.01 to 500 mg/Kg body weight preferably in the range of 0.05 to 250 mg/Kg body weight and most preferably in the range of 0.15 to 50 mg/kg body weight. The oligonucleotide analogues do not rapidly enter cells. To improve cell entry, the oligonucleotide can be delivered together with a membrane permeablizing agent like polyoxyethylene sorbitan mono-oleate (Tween 80) or polyethylene glycol 300 molecular weight (PEG 300). Alternatively, the oligonucleotide solutions can be prepared by including it as an encapsulated material in a liposome suspension by any of several techniques. Alternatively, oligonucleotides are conjugated to compounds which expand the lifetime of the material in the circulating blood and enhance endothelial cell uptake. An example of a suitable conjugation moiety would be the vitamin folate.

Obviously, many modifications and variations of the present invention are possible in light of the above teachings. It is therefore to be understood that, within the scope of the appended claims, the invention may be practiced otherwise than as specifically described.

    __________________________________________________________________________     SEQUENCE LISTING                                                               (1) GENERAL INFORMATION:                                                       (iii) NUMBER OF SEQUENCES: 15                                                  (2) INFORMATION FOR SEQ ID NO:1:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:                                        GCTTTCCCGGAAACCTCGCGC21                                                        (2) INFORMATION FOR SEQ ID NO:2:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:                                        GGGGCGGTGCTGCTTTCCCGG21                                                        (2) INFORMATION FOR SEQ ID NO:3:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:                                        AGCCACCTGGGGGCCAAGGGG21                                                        (2) INFORMATION FOR SEQ ID NO:4:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:                                        TGATCCTTTATAGCGCTAGCC21                                                        (2) INFORMATION FOR SEQ ID NO:5:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:                                        GCGTCGACTGGGGCGCGTGAT21                                                        (2) INFORMATION FOR SEQ ID NO:6:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:                                        CTCTGAGTAGCAGAGGAGCTC21                                                        (2) INFORMATION FOR SEQ ID NO:7:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:                                        CCCCGACTCACCTGGGAACAG21                                                        (2) INFORMATION FOR SEQ ID NO:8:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:                                        GCTACACATGTCTATGGAGGG21                                                        (2) INFORMATION FOR SEQ ID NO:9:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:                                        GGCAGAAATGTATGTGGGTGG21                                                        (2) INFORMATION FOR SEQ ID NO:10:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:                                       ACACATACACACACACACACA21                                                        (2) INFORMATION FOR SEQ ID NO:11:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:                                       AGCCATAGCGAGGCTGAGGTT21                                                        (2) INFORMATION FOR SEQ ID NO:12:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 15 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:                                       GGTTGCAACTCTGAG15                                                              (2) INFORMATION FOR SEQ ID NO:13:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:                                       GCCATAGCGAGGCTGA16                                                             (2) INFORMATION FOR SEQ ID NO:14:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 17 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:                                       CTATGGAGGGCCACTTC17                                                            (2) INFORMATION FOR SEQ ID NO:15:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 18 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (iii) HYPOTHETICAL: NO                                                         (iv) ANTI-SENSE: YES                                                           (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:                                       GGGCCACTTCTTCTGTAA18                                                           __________________________________________________________________________ 

What is claimed is:
 1. An oligonucleotide having a nucleotide sequence which comprises between about 15 to 30 base, and is complementary to a sequence contained in the pre-mRNA or mature mRNA transcript of human ICAM-I, said oligonucleotide selected from the group consisting of:GCT TTC CCG GAA ACC TCG CGC (SEQ ID No. 1); CTC TGA GTA GCA GAG GAG CTC (SEQ ID No. 6); CCC CGA CTC ACC TGG GAA CAG (SEQ ID No. 7); GCT ACA CAT GTC TAT GGA GGG (SEQ ID No. 8); GGC AGA AAT GTA TGT GGG TGG (SEQ ID No. 9); ACA CAT ACA CAC ACA CAC ACA (SEQ ID No. 10); AGC CAT AGC GAG GCT GAG GTT (SEQ ID No. 11); GGT TGC AAC TCT GAG (SEQ ID No. 12); CTA TGG AGG GCC ACT TC (SEQ ID No. 14), and GGG CCA CTT CTT CTG TAA (SEQ ID No. 15).
 2. An oligonucleotide according to claim 1 wherein the oligonucleotide has the sequence 5'-AGC CAT AGC GAG GCT GAG GTT-3'(SEQ ID No. 11). 